In contrast, nucleotide polymorphisms were observed between the species belonging to the same genus and the average Selleck JQ1 of interspecific divergence (4.2–11%) was much more significant than the intraspecific divergence. This contrasts with studies on Aspergillus species, which show that the intra- and interspecific diversities were at the same level and prevent the species boundaries (Geiser et al.,
2007). Interestingly, the rate of interspecific divergence was not related to the ability of species discrimination by the cox1 sequence because the species of the genera characterized by a low rate were completely discriminated. This low divergence could be explained by a recent speciation leading to the slow evolution of the cox1 gene. The nucleotide variations of the partial cox1 gene are sufficient to discriminate all the studied species in accordance with the results observed in the Animal Kingdom, in which >96% of species have been discriminated (Hebert et al., 2004; Garcia-Valera & Nadler, 2006; Hajibabaei et al., 2006). The cox1 gene has been compared with the SSU-rDNA and the ITS sequences. The analysis of the SSU-rDNA Alectinib purchase revealed a high conservation of the nucleotide sequences between the species, allowing the resolution of only 52% of species. This result can be compared with the reported analysis in flowering plants in which only a few base pairs of nucleotide divergence have been observed
(Cho et al., 2004), Plasmin and suggests that the fungal SSU-rDNA genes are under selective pressure, which prevents numerous mutation events. The only genera in which the species
were well discriminated concerned those with no more than three species investigated. When comparing the ITS sequences within each genus, the rates of nucleotide divergences were similar to those obtained with the cox1 gene, and yet the species discrimination rates were lower. Indeed, in the genus Cladosporium, among the five species studied, no species had specific ITS sequences. Two species, on the one hand, and three, on the other, had a divergence of at least five and 24 nucleotides, respectively, with the cox1 gene, each shared an identical sequence. To confirm this high conservation of the ITS within this genus, nine species for which the ITS sequences are available in the GenBank database were investigated and only two types of sequences were found between these species. Moreover, although in other genera, we have highlighted a high divergence between the ITS sequences, it is mainly the insertions/deletions that prevent the alignment of these sequences and a phylogenetic analysis among distant species belonging to different fungal phyla. The survey of the cox1 sequences showed that among 47 isolates investigated, only four (9%) shared intronic sequences possessing significant similarities to the introns described in the phylogenetically distant species. All these introns are mobile elements that encode the Homing endonucleases.