6e-05). However, although inactivation of crc alleviated repression of OprB1 on 0.8% glucose medium, the OprB1/OprF ratio was still higher on 0.2% glucose medium (Figure 7D, compare results for the crc mutant on 0.2 and 0.8% glucose, p = 6.7e-04). Therefore we conclude that in addition to the Crc some other factor(s) as yet unknown should be implicated in hunger-induced up-regulation of OprB1. Figure 7 Post-transcriptional
regulation of OprB1 depends selleck screening library on the glucose concentration. A. β-Galactosidase (β-Gal) activity expressed from the gtsA promoter was measured in the wild-type P. putida grown on solid medium with 0.2 or 0.8% glucose or 0.2% gluconate. B. SDS-PAGE of the outer membrane protein preparations from P. putida wild-type PaW85 (wt) and from OprB1-overexpressing strain PaWoprB1-tacB1 (B1tacB1) grown 24 hours over the whole Petri plate. The growth medium contained 0.2 or 0.8% glucose (glc) as a carbon source. Plus (+) mark above the lane indicates that the bacterial growth medium contained also 0.5 mM IPTG. C and D. Analysis of the effect of the crc inactivation on the hunger-induced up-regulation
of OprB1. The outer membrane proteins were prepared from P. putida wild-type click here (wt) and crc mutant strains (crc) grown for 24 hours as a lawn over the entire Petri plate. The growth medium contained 0.2 or 0.8% glucose (glc). The ratio of OprB1 to OprF was calculated from the data of at least two independent
protein preparations and five independent gel runs. Mean values and 95% confidence intervals are presented. Discussion Previous studies on ColRS signaling system have revealed a peculiar Erastin cost subpopulation lysis GDC-0449 clinical trial phenotype of the colR mutant grown on glucose solid medium . In this study we clarified the reasons for glucose-specific cell lysis and revealed that the ColRS system is necessary for P. putida to survive the hunger response which includes up-regulation of sugar channel OprB1. Several lines of evidence obtained in this study suggest that the glucose-growing colR mutant experiences envelope stress caused by the accumulation of membrane proteins. This was first indicated by the collection of mutants suppressing the lysis phenotype of the colR-deficient strain. These data demonstrated that the loss of ColR can be suppressed by down-regulation of certain OM proteins like OprB1 and OprF, as well by hindering the SecB-dependent protein secretion. Second, artificial overexpression of sugar channel protein OprB1 further highlighted the specifically increased sensitivity of the colR mutant to this particular OM protein.