zeae. Deletion of PDC1 reduces lipid accumulation in the aerial but not the embedded mycelia. This suggests that the PAA pathway is the only pathway that produces lipids for the aerial mycelia and that PDC1-dependent lipid production is important for perithecia maturation. Additionally, PDC1 is required for vegetative growth of the embedded mycelia. Although lipid accumulation in the aerial mycelia was markedly reduced in the PDC1 deletion mutant,

the total amount of lipids was not significantly different compared with the wild-type strain (Fig. 2 and Fig. S4). This is unexpected, given that lipids from the aerial C59 wnt manufacturer mycelia constitute about 20% of the total lipid content in the carrot agar culture (Son et al., 2011). One possible explanation for this discrepancy could be that higher lipid concentrations in the densely embedded mycelia of PDC1 deletion mutants may compensate H 89 cost for the lower lipid accumulation in the aerial mycelia. Other enzymes, such as carnitine acetyl transferases (CATs), ACL, and acetyl-CoA hydrolase, could also be compensating for reduced lipid production in the embedded mycelia (Fig. S5). ACS1 is a downstream enzyme of PDC1 in PAA pathway and known to be required for POL production (Lee et al., 2011). The PDC1 deletion repressed

ACS1 expression, although the ACS1 deletion did not suppress PDC1 expression. This suggests that the ACS1 deletion mutant must be accumulating toxic PAA pathway Rebamipide intermediates such as acetate, acetaldehyde, and ethanol. As ACS1 is crucial for ridding the fungal cells of these toxic compounds (Lee et al., 2011), the ACS1 deletion strain might be expected to demonstrate more severe

defects than the PDC1 deletion strain. The less severe phenotypes observed for the double mutant compared with the ACS1 deletion mutant support our hypothesis. Active fermentation pathways are commonly found in eukaryotes under both aerobic and anaerobic conditions. Plants also used PAA pathway for hypoxic growth of waterlogged root and also for other specific conditions such as seed growth and pollen tubes elongation (Peschke & Sachs, 1993; Gass et al., 2005). In filamentous fungi, PDC is regarded as an important postglycolytic enzyme in N. crassa under aerobic conditions and is closely associated with ethanol production in A. nidulans (Alvarez et al., 1993; Lockington et al., 1997). Similarly, G. zeae seems to utilize PDC1-dependent metabolic pathways for normal aerobic growth and possibly for ethanol fermentation. Aerial mycelia take nutrients from embedded hyphae for growth in obligate heterotrophic fungi. Nutrient translocation mechanisms are well studied in arbuscular mycorrhizal (AM) fungi, which utilize triacylglycerol to translocate carbon sources absorbed from host plants to the extraradical mycelium (Bago et al., 2000, 2002; Lammers et al., 2001; Parniske, 2008).