Using the delocalization
and charge transfer descriptors, we obtain all couplings between these three states. Our results are important in the context of DNA photophysics, since the calculated couplings can be used to parametrize effective Hamiltonians to model extended DNA stacks. Our calculations Bioactive Compound Library screening also suggest that the 5′-purine-pyrimidine-3′ sequence favors the formation of charge transfer excited states. (C) 2014 AIP Publishing LLC.”
“Human detection is a significant and challenging task with applications in various domains. In real-time systems, the speed of detection is crucial to the performance of system, while the accuracy is also taken into consideration. In this work, a human detection approach based on Histograms of Oriented Gradients (HOG) feature and differential evolution (DE), termed as HOG-SVM-DE, is proposed to achieve both fast and accurate detection. The proposed method considers the problem of locating an objective detection window as a search problem, and speeds up the detection stage by solving the search problem with DE. DE is chosen as the optimizer as it is characterized by fast and global convergence. The proposed system trains only one linear-SVM, and allows tradeoffs between the detection rate and the detection
time to satisfy different applications by simply tuning one parameter. Experiments are conducted on a set of images from the INRIA Person Dataset, and the results validate that the proposed HOG-SVM-DE is promising in terms of both speed and accuracy. (C) 2014 Elsevier B.V. All rights reserved.”
“Background IgE binds to mast cells and basophils via
its high-affinity GSK1120212 supplier receptor, GM6001 concentration Fc epsilon RI, and cross-linking of Fc epsilon RI-bound IgE molecules by allergen leads to the release of allergic mediators characteristic of type I hypersensitivity reactions. Previous work has shown that cross-linking of Fc epsilon RI with Fc gamma RIIb, an ITIM-containing IgG receptor, leads to inhibition of basophil triggering. 2G10, a chimeric human IgG1 anti-idiotype, has broad reactivity with human IgE and as such has the potential to bind simultaneously to Fc epsilon RI-bound IgE, via its Fab regions, and the negative regulatory receptor, Fc gamma RIIb, via its Fc region.\n\nObjective To assess the ability of human 2G10 to inhibit anti-IgE and allergen-driven basophil degranulation through cross-linking of Fc epsilon RI-bound IgE with Fc gamma RIIb.\n\nMethods 2G10 was assessed for its ability to bind to Fc gamma RIIb on transfected cells and on purified basophils. In the basophil degranulation assay, basophils were purified from peripheral blood of atopic individuals and activated with either anti-IgE or the house dust mite allergen Der p 1, in the presence or absence of human 2G10. Basophil activation was quantified by analysis of CD63 and CD203c expression on the cell surface, and IL-4 expression intracellularly, using flow cytometery.