These results directly link DLEU2 with TRIM13 transcriptional regulation in the presence of HBx. In silico analysis indicates that DLEU2 RNA species potentially binds the HBx protein and we confirmed the HBx-DLEU2 interaction using an anti-HBx RNA Immune Precipitation (RIP). Finally, we found that DLEU2 inac-tivation has a profound impact on HBV pgRNA transcription, thus unveiling a functional relevance of the DLEU2-HBx interaction in regulating HBV replication. Conclusion: Genome wide occupancy study revealed that HBx targets 39 lncRNA
promoters. HBx binding to the DLEU2 promoter modifies its splicing profile and affects the expression of the intragenic/overlapping TRIM13 gene, hsa-mir-15 and hsa-mir-16. Moreover, a direct interaction of DLEU2
with the HBx regulates HBV replication. Disclosures: Massimo Levrero – Advisory Committees or Review Panels: Gilead, Jansen Cilag; Speaking and Teaching: Roche, BMS, MSD The following buy ICG-001 people have nothing to disclose: Francesca Guerrieri, Safaa Jed-dari, Daniel D’Andrea, Anna Tramontano Backgrounds and Aims: Fc gamma receptors (FCGRs) are important in regulating immune responses. Most of the FCGRs are activating receptors including FCGR1, FCGR2A, FCGR3, whereas FCGR2B is the only inhibiting S1P Receptor inhibitor receptor. So far, there is no FCGRs study regarding hepatitis B virus (HBV) infection. Methods: whole-genome expression profiling of purified RNA of peripheral blood mononuclear cells (PBMC) from patients with acute self limiting hepatitis B (AHB) and treatment naive chronic HBV infection including immune tolerant(IT) and HBeAg positive(HBeAg+) chronic hepatitis B(CHB)and healthy control (HC) was performed using microarrays. Gene signatures Fenbendazole were developed through bioinformatics approaches and further evaluated and validated by real time RT-PCR and western blot. Results: Microarray data showed activating FCGRs namely FCGR1A and FCGR2A that were differentially expressed and validated by real time RT-PCR in the same patient
cohort, which revealed higher expression level of FCGR1A and FCGR2A in AHB compared with chronic HBV infection, in HBeAg+ CHB compared with IT and in AHB compared with HC(all, P<0.001). Subsequently studies with an independent patient cohort of 25 AHB, 40 IT, 50 HBeAg+ CHB showed that FCGR1A and FCGR2A had the similar expression pattern which was most significant for FCGR1A mRNA expression(P<0.001) and protein expres-sion(p<0.01), whereas FCGR3A expression was statistically insignificant. Also, we assessed mRNA expression of FCGR2B which revealed higher expression in IT compared with HBeAg+ CHB without statistical significance. The ratio of FCGR2A/ FCGR2B mRNA expression was calculated in 10 HBeAg+ CHB(10/50) before and after PEG-IFN therapy who achieved virological response. The result showed that the ratio FCGR2A/ FCGR2B was higher in those after treatment compared with those before treatment(5.88±5.12 vs 2.05±1.86, p<0.01).