In total, PI3K Inhibitor Library we added 290 new BP terms to the GO for 48 secondary metabolites produced by one or more Aspergillus species. There are over 400 Aspergillus genes in AspGD that have been manually or computationally annotated to more specific secondary metabolism BP terms, based on over 260 publications (Table 2). A complete list of the GO terms for secondary metabolic process annotations is available in Additional file 1. The addition of new terms is ongoing as new secondary metabolites and their biosynthetic genes are identified and described in the scientific literature. The process of adding new GO terms depends on the elucidation of the structure of the secondary
metabolite as the structure is required for new ChEBI (Chemical Entities of Biological Interest; http://www.ebi.ac.uk/chebi/) terms to be assigned, and these chemical compound terms are a prerequisite for GO term assignments involving chemical compounds. These new and improved GO terms provide researchers with valuable clues to aid in the identification of proteins involved in the production of specific classes of Aspergillus secondary metabolites. Table 2 GO terms used for secondary metabolism annotations at AspGD A. nidulans A. fumigatus A. niger A. oryzae Number of predicted protein-encoding genes
10,287 9,793 13,870 11,896 Number of genes with GO annotations to secondary metabolism 248 171 228 195 Number of genes with manual GO annotations to secondary metabolism* 202 96 81 32 Number of genes with computational GO annotations to secondary metabolism* 58 98 Opaganib 170 166 * or to child terms of ‘secondary metabolic process’ (GO: 0019748). Predictive annotation using orthology relationships in conjunction with experimentally-based GO term assignments Manual curation of the
genes of one species can be used to computationally annotate the uncharacterized genes in another species based on orthology relationships. The use of GO to describe gene products facilitates comparative analysis of functions of orthologous genes throughout the tree of life, including orthologous genes within the filamentous check fungi. To augment the manual GO curation in AspGD, we leveraged orthology relationships to assign GO annotations to genes that lacked manual annotations of their own but which had an experimentally characterized ortholog in AspGD, the Saccharomyces Genome Database (SGD) (http://www.yeastgenome.org) or PomBase (http://www.pombase.org). A total of 492 GO annotations were made to secondary metabolism-related genes in A. nidulans, A. fumigatus, A. niger and A. oryzae based on their orthology relationships (Table 3). Files listing these orthology relationships are available for download at http://www.aspergillusgenome.org/download/homology/orthologs/ and the files describing all GO term annotations for each gene product in AspGD are available at http://www.aspergillusgenome.org/download/go/.