(C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 120: 1490-1495, 2011″
“Materials and methods: Rats were exposed to a 6 Gy dose irradiation from a cobalt<SU60</SU source. Whole-cell potassium current was measured in freshly isolated rat aorta smooth muscle cells using standard patch-clamp technique.

Results: We have determined that whole-body ionising irradiation significantly inhibits whole-cell outward K<SU+</SU current in rat aortic VSMC obtained from irradiated rats 9 and 30 days after irradiation, and

this inhibition appears to be increased throughout post-irradiation period. Using selective inhibitors of small conductance Ca<SU2+</SU-activated K<SU+</SU channels (SKCa), apamin AZD5363 price (1 mu M), intermediate conductance Ca<SU2+</SU-activated

K<SU+</SU channels (IKCa,), charybdotoxin (1 mu M) and a large conductance Ca<SU2+</SU-activated K<SU+</SU channels (BKCa), paxilline (500 nM), we established that the main component of whole-cell outward K<SU+</SU current in rat aortic VSMC is due to BKCa. It is clear that PRIMA-1MET on the 9th day after irradiation paxilline had only a small effect on whole-cell outward K<SU+</SU current in VSMC, and was without effect on the 30th day post-irradiation, suggesting complete suppression of the BKCa current. The PKC inhibitor, chelerythrine (100 nM), effectively reversed the suppression of whole-cell outward K<SU+</SU current induced by ionising irradiation in the post-irradiation period of 9 and 30 days.

Conclusions: The results suggest that irradiation-evoked inhibition of the BKCa current in aortic VSMC is mediated by PKC. Taken together, our data indicate that one of the mechanisms leading to elevation of vascular tone and related arterial hypertension development under ionising irradiation impact is a PKC-mediated inhibition of BKCa channels in VSMC.”
“The crystallographic, magnetic

and BKM120 PI3K/Akt/mTOR inhibitor electronic structures of the ferromagnetic shape memory alloys Ni2XGa (X=Mn, Fe, and Co), are systematically investigated by means of the first-principles calculations within the framework of density functional theory using the VIENNA AB INITIO SOFTWARE PACKAGE. The lattice parameters of both austenitic and martensitic phases in Ni2MnGa have been calculated. The formation energies of the cubic phase of Ni2XGa are estimated, and show a destabilization tendency if Mn atom is substituted by Fe or Co. From Ni2MnGa to Ni2CoGa, the down spin total density of states (DOS) at Fermi level is gradually increasing, whereas that of the up spin part remains almost unchanged. This is the main origin of the difference of the magnetic moment in these alloys. The partial DOS is dominated by the Ni and Mn 3d states in the bonding region below E-F.