Serum TBARS was estimated in thirty nine newly diagnosed drug free OCD patients and thirty three disease free control subjects. Mean values for serum TBARS were found to be significantly higher (P < 0.001) in cases than in controls (5.85 nmol/ml and 3.90 nmol/ml with an SD of 0.56 and 0.81 respectively). A strong positive correlation (r(s)=0.757, p < 0.01) between the lipid peroxidation marker TBARS and the disease severity indicator
YBOCS was found among cases. Significant positive correlation was also found between TBARS and the obsessive and compulsive subscales of YBOCS. These Sapitinib mw findings were in tune with previous studies, which suggested oxidative stress induced increased free radical generation in the OCD patients. Our findings may help in understanding the development and progress of OCD and the treatment of patients of OCD in future. (c) 2009 Elsevier Tanespimycin mw Inc. All rights reserved.”
“Diaminofluorescein-dyes (DAFS) are widely used for visualizing NO center dot production in biological systems. Here it was examined whether
DAF-fluorescence could be evoked by other means than nitrosation. Tobacco (Nicotiana tabacum) suspension cells treated with the fungal elicitor cryptogein released compound(s) which gave a fluorescence increase in the cell-free filtrate after addition of DAF-2 or DAF-FM or DAR-4M. DAF-reactive compounds were relatively stable and identified as reaction products of H2O2 plus apoplastic peroxidase (PO). CPTIO prevented formation of these products. Horseradish-peroxidase (HR-PO) plus H2O2 also generated DAF-fluorescence in vitro. Using RP-HPLC with fluorescence detection. DAF derivatives were further analyzed. In filtrates from cryptogein-treated cells, fluorescence originated from two novel DAF-derivatives also obtained in vitro with DAF-2 + HR-PO +
H2O2. DAF-2T was only detected when an NO donor (DEA-NO) was present. Using high resolution mass spectrometry, the two above-described PRT062607 novel DAF-reaction products were tentatively identified as dimers.
In cells preloaded with DAF-2 DA and incubated with or without cryptogein, DAF-fluorescence originated from a complex pattern of multiple products different from those obtained in vitro. One specific peak was responsive to exogenous H2O2, and another, minor peak eluted at or close to DAF-2T.
Thus, in contrast to the prevailing opinion, DAF-2 can be enzymatically converted into a variety of highly fluorescing derivatives, both inside and outside cells, of which none (outside) or only a minor part (inside) appeared NO center dot dependent. Accordingly, DAF-fluorescence and its prevention by cPTIO do not necessarily indicate NO center dot production. (C) 2012 Elsevier Inc. All rights reserved.”
“Transcriptional induction of beta interferon (IFN-beta) through pattern recognition receptors is a key event in the host defense against invading viruses.