Results: The non-selective Kv7.2-5 channel positive allosteric modulators (PAMs) flupirtine and retigabine reduced the SIH response and basal body temperature with concomitant locomotor sedation. Administration of the Kv7.4/Kv7.5 channel negative allosteric modulator R-BMS204352 prior to injection with flupirtine did not antagonize the effects on body temperature and locomotor activity. Administration of Ricolinostat the Kv7.4/Kv7.5 preferential PAM S-BMS204352 only modestly affected basal body temperature but did not affect the SIN response or locomotor activity levels.
Conclusions:
The present study supports a role of Kv7.2/Kv7.3 channels in anxiety reduction, hypothermia and sedation, whereas preferential activation of Kv7.4/Kv7.5 channels only modestly affected body temperature and locomotor activity. Thus, the effects of Kv7 channel openers may be dissociated with regard to the contribution of different Kv7 subunits and Kv7 channel isoforms. (C) 2011 Elsevier Ltd. All rights reserved.”
“Many proteomic studies Selleck LB-100 focus
on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy-to-use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compatible with all available 4-plex isobaric tags. jTraqX can be freely downloaded at http://sourceforge.net/projects/protms.”
“Interstrain Tau-protein kinase differences in thermoregulation of rats are important in biomedical research because subtleties in thermoregulatory sensitivities
may greatly affect data collected. Little is known regarding how individual rodent strains differentially utilize behavioral thermal preference to regulate core temperature (Tc). Sprague-Dawley (SD) and Fischer 344 (F344) rats are known to have differences in thermoregulation including heat tolerance and are useful models to study interstrain differences in thermoregulation. Adult male SD and F344 rats of similar body size were implanted with radiotelemetry thermoprobes (DS!) to measure Tc and MA and housed in either a longitudinal temperature gradient with an ambient temperature (Ta) range of similar to 15-40 degrees C to measure selected Ta (STa) or control environment maintained at a Ta of 23 degrees C. When continuously monitored for 48 h, Tc and MA increased at night, while STa decreased, according to their normal circadian cycle in both strains. SD rats were more active than F344 rats throughout the circadian cycle (SD gradient: day =12.9 1.2 m/h, night = 32.1 +/- 2.4 m/h; F344 gradient: day = 4.1 +/- 0.6 m/h, night = 16.8 +/- 1.8 m/h; p < 0.05 interstrain and circadian effects). The STa of each strain was greater during the daytime (SD: 26.4 +/- 0.2 degrees C; F344: 27.8 +/- 0.3 degrees C) than at night (SD: 24.7 +/- 0.3 degrees C; F344: 25.7 +/- 0.