Concentrations of oxidants used were based on the amounts {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| necessary to eradicate CFU viability as assessed in the previous experiments. A) All organisms displayed significant NVP-BSK805 chemical structure reduction in ATP production (One-way ANOVA) in an H2O2 dose-dependent manner up to 5 mM. B) ATP production by KP was statistically unaffected by HOCl exposure up to 0.1 mM according to one-way ANOVA (p = 0.53) while all other organisms tested displayed significant HOCl dose-dependent reduction in ATP production in this concentration range.
Error bars represent standard deviation of at least n = 3 experiments. Figure 6 Correlating H 2 O 2 -induced loss of ATP production with bacterial viability. H2O2-induced disruption of ATP production correlated statistically with abolishment of CFU viability for all organisms tested except PsA (p = 0.15) at concentrations up to 5 mM. Though the decline of ATP production in PsA for this oxidant was statistically significant
in this range, the percent FG-4592 in vitro change remains independent of the percent reduction in CFU viability. Solid circles and lines: ATP recovery after oxidant exposure. Open circles and dotted lines: CFU viability. Both parameters are measured as percent relative to oxidant-free controls. P-values represent linear regression of the raw data values from percent ATP recovery versus CFU viability. Values less than 0.05 were considered significant and denote correlation between the parameters; values greater than 0.05 indicate independence of the parameters. Error bars represent standard deviation of at least n = 3 experiments. ATP production was dose-dependently abolished in PsA, SA, BC, and EC while KP remained statistically unaffected even at HOCl doses up to 0.1 mM (PsA, p < 0.0001; SA, p < 0.0001; BC, p < 0.0001; EC, p < 0.0001 and KP, p = 0.53; Figure 5B). The decline in ATP production correlated with HOCl-induced loss of CFU viability in PsA, BC, and EC (p = 0.005, 0.006, and 0.01, respectively, Figure 7) but was independent
of diminished CFU viability in SA and KP (p = 0.20 and 0.60, respectively). Figure 7 Correlating HOCl-induced ATP changes with bacterial viability. ATP production is affected by HOCl exposure and correlates statistically with CFU viability in PsA, BC, and EC (p = selleck chemicals 0.005, 0.006, and 0.01, respectively); however, SA and KP lose CFU viability after exposure to lower concentrations of HOCl than are required to abolish ATP production during the assay time. Solid circles and lines: ATP recovery after oxidant exposure. Open circles and dotted lines: CFU viability. Both parameters are measured as percent relative to oxidant-free controls. P-values represent linear regression of the raw data values from percent ATP recovery versus CFU viability. Values less than 0.05 were considered significant and denote correlation among the parameters; values greater than 0.05 indicate independence of the parameters. Error bars represent standard deviation of at least n = 3 experiments.