, 2010). These profound changes in cortical network dynamics also correlate with dramatic changes in sensory processing (Fanselow and Nicolelis, CFTR modulator 1999, Castro-Alamancos, 2004, Hentschke

et al., 2006, Crochet and Petersen, 2006 and Ferezou et al., 2007). It is therefore of crucial importance to study Vm dynamics in awake animals actively sensing and exposed to natural stimuli. Here, through whole-cell Vm recordings of layer 2/3 pyramidal neurons in the mouse barrel cortex, we investigate how tactile information from a single whisker (C2) is processed during active touch. Sensory information relating to the C2 whisker is signaled to the C2 barrel column of primary somatosensory cortex, an anatomically defined region of the mouse brain with a diameter of approximately 250 μm containing around 6500 neurons (Lefort et al., 2009). Investigations of this specific cortical column have begun to yield quantitative information relating to its synaptic structure (Knott et al., 2002), synaptic connectivity (Lefort et al., 2009), and functional operation

during behavior (Crochet and Petersen, 2006, Poulet and Petersen, 2008 and Gentet et al., 2010). The convergence of techniques focusing upon a single well-defined cortical column may help toward a quantitative RAD001 and mechanistic understanding of how a specific neocortical microcircuit processes sensory information. Whole-cell recordings were obtained from head-restrained mice and the Vm dynamics of layer 2/3 neurons located in the C2 barrel column were correlated with C2 whisker-related behavior through high-speed filming (500 Hz) under infrared illumination (Figures 1A and 1B). Objects could be inserted on the millisecond timescale into the trajectory of the C2 whisker in one of two different locations using piezoactuators (schematically indicated as red and blue objects in Figure 1A). The C2 whisker-related Cell press behavior was quantified off-line based on the high-speed filming (Figure 1C; Movies

S1 and S2 available online). We distinguished between three different behavioral periods (Figures 1B and 1C): free whisking (W, when both piezoactuators were raised up and the whisker moved back and forth freely without touching any object); active touch (T, when one of the piezoactuators was lowered and the mouse actively moved the C2 whisker repetitively against the object causing a bending of the whisker); and quiet wakefulness (Q, when the awake mouse was not moving its whisker). The recorded neurons were labeled with biocytin for post-hoc anatomical identification and location relative to the barrel map (Figure 1D). Membrane potential dynamics evoked by C2 whisker touch (Figure 1E) were compared with periods of free whisking and quiet waking.