c) injection of mercuric chloride (HgCl2) and the participation of the autonomic nervous system in these responses. 58 animals were utilized and distributed randomly into 10 groups and administered a 5 mu L intracisternal injection of 0.68 mu g/kg HgCl2 (n = 7), 1.2 mu g/kg HgCl2 (n = 7), 2.4 mu g/kg HgCl2 (n = 7), 60 mu g/kg HgCl2 (n = 7), 120 mu g/kg HgCl2 (n = 3), saline (control) (n = 7), 60 mu g/kg HgCl2 plus prazosin (n = 6),

saline plus prazosin (n = 6), 60 mu g/kg HgCl2 plus DMH1 metilatropina (n = 4) or saline plus metilatropina (n = 4)HgCl2. Anesthesia was induced with halothane and maintained as needed with urethane (1.2 g/kg) administered intravenously (i.v.) through a cannula placed in the left femoral vein. The left femoral artery was also cannulated to record systolic arterial pressure (SAP), diastolic arterial pressure (DAP) and heart rate (HR). A tracheotomy was performed to record respiratory rate. Animals were placed in a stereotaxic frame, and the cisterna magna was exposed. After a stabilization period, solutions (saline or HgCl2)

were injected and cardiopulmonary responses were recorded for 50 min. Involvement of the autonomic nervous system was assessed through the i.v. injection of hexamethonium (20 mg/kg), prazosin (1 mg/kg) QNZ chemical structure and methylatropine (1 mg/kg) 10 min before the i.c. injection of HgCl2 or saline. Treatment with 0.68, 1.2, 2.4 mu g/kg HgCl2 or saline did not modify basal cardiorespiratory parameters, whereas the 120 mu g/kg dose induced acute toxicity, provoking respiratory arrest and death. The administration of 60 mu g/kg HgCl2, however, induced significant increases (p < 0.05) in SAP at the 30 degrees, 40 degrees and 50 degrees min, timepoints and DAP at the 5 degrees, 10 degrees, 20 degrees, 30 degrees, 40 degrees and 50 degrees timepoints. RR was significantly decreased at the Ganetespib chemical structure 5 degrees, 10 degrees, 20 degrees, 40 degrees and 50 degrees min timepoints; however, there was no change in HR. Hexamethonium administration, which causes non-specific inhibition of the autonomic nervous system, abolished the observed cardiorespiratory effects. Similarly, prazosin, a al-adrenoceptor blocker that specifically

inhibits sympathetic nervous system function, abolished HgCl2 induced increases in SAP and DAP without affecting HR and RR. Methylatropine (1 mg/Kg), a parasympathetic nervous system inhibitor, exacerbated the effects of HgCl2 and caused slow-onset respiratory depression, culminating in respiratory arrest and death. Our results demonstrate that increases in SAP and DAP induced by the i.c. injection of mercuric chloride are mediated by activation of the sympathetic nervous system. (C) 2011 Elsevier Inc. All rights reserved.”
“In recent years, the potential value of the non-structural proteins (NSP) 2C and 3ABC has been well documented for differentiation of animals infected with foot-and-mouth disease virus (FMDV) from vaccinated animals (DIVA).